soluble cd40 ligand (scd40l) elisa kit  (R&D Systems)

Journal: Clinical and Applied Thrombosis/Hemostasis

Article Title: Risk of Atherosclerosis Due to HMGB1-dependent Platelet-derived Microparticles in Patients with Type 2 Diabetes Mellitus

doi: 10.1177/10760296241302082

Figure Lengend Snippet: Multivariate Analysis of HMGB1 in Patients with T2DM.

Article Snippet: ELISA kits for soluble vascular cell adhesion molecule (sVCAM-1), plasminogen activator inhibitor (PAI-1), interleukin-6 (IL-6), soluble E-selectin (sE-selectin), regulated on activation, normal T cell expressed and secreted (RANTES), soluble P-selectin (sP-selectin), soluble CD40 ligand (sCD40L) and monocyte-chemoattractant protein (MCP)-1 were purchased from BioSource International, Inc. (Camarillo, California, USA).

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antiplatelet Activity of Tetramethylpyrazine via Regulation of the P2Y12 Receptor Downstream Signaling Pathway

doi: 10.1155/2022/7941039

Figure Lengend Snippet: Effect of SQ22536 and LY294002 on the release of sCD40L and IL-1 β . Results are expressed as mean ± SD. n = 6. & P < 0.05 vs. resting; ∗ P < 0.05 vs. control; # P < 0.05 vs. model; + P < 0.05 vs. TMP; ▲ P < 0.05 vs. SQ22536; △ P < 0.05 vs. LY294002.

Article Snippet: The IL-1 β and soluble CD40 ligand (sCD40L) ELISA kits were obtained from Elabscience Biotechnology Co., Ltd. (Wuhan, China).

Techniques: Control

Journal: PLoS ONE

Article Title: An Autocrine Cytokine/JAK/STAT-Signaling Induces Kynurenine Synthesis in Multidrug Resistant Human Cancer Cells

doi: 10.1371/journal.pone.0126159

Figure Lengend Snippet: A. The cDNA from A549 and A549/dx cells was analyzed by a PCR array specific for IL-6/STAT3 signaling, as reported under Materials and methods. The fold regulation of the 83 genes analyzed, expressed in logarithmic scale, was represented in a colorimetric scale. The figure is the mean of 4 experiments. B. The levels of IL-6, IL-4, IL-1β, IL-13, CD40L, IFN-γ were measured in the cell culture supernatants by specific ELISAs. Data are presented as means ± SD (n = 3). * p < 0.02, ** p < 0.005, *** p < 0.001: A549/dx cells versus A549 cells.

Article Snippet: The production of cytokines was measured in the cell culture supernatant using the following commercial kits: Human interleukin-6 (IL-6) Duo Set Development Kit (R&D Systems), Human interleukin-4 (IL-4) DuoSet Development Kit (R&D Systems), Human IL1-beta (IL-1β) platinum ELISA kit (eBioscience, San Diego, CA), Human interleukin-13 (IL-13) ELISA Development Kit (Peprotech, London, UK), Human soluble CD40 Ligand (sCD40L) ELISA Development Kit (Peprotech), Human tumor necrosis factor- α (TNF-α) DuoSet Development Kit (R&D Systems), Human IFN-γ DuoSet Development Kit (R&D Systems).

Techniques: Cell Culture

Journal: Critical Care

Article Title: Age of blood and recipient factors determine the severity of transfusion-related acute lung injury (TRALI)

doi: 10.1186/cc11178

Figure Lengend Snippet: Supernatant composition

Article Snippet: Proteins that appeared to increase with storage were then quantified by commercial ELISA kits for EGF, ENA-78, GRO-α, IL-8, IL-16, and MCP-1 (R&D Systems, Minneapolis, MN, USA), and also for soluble CD40 ligand (sCD40L) (Bender MedSystems, Vienna, Austria) according to the manufacturers' instructions.

Techniques:

Journal: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Article Title: The CD40-CD154 co-stimulation pathway mediates innate immune injury in adriamycin nephrosis.

doi: 10.1093/ndt/gfp569

Figure Lengend Snippet: Fig. 4. CD40 expression in mouse kidney. Immunohistochemical staining of frozen kidney sections demonstrated marked upregulation of CD40 expression in ADR+IgG mice (B) compared with ADR+MR1 (C) and normal mice (A). Quantitation was performed by a blinded observer by counting the number of stained cells per high power field in at least five sections per mouse (D). To identify which cells express CD40, kidney sections were stained for F4/80 (primary ab: rat anti-mouse F4/80, secondary ab: rhodamine conjugated rabbit anti-rat IgG) and CD40 (primary ab: goat anti-mouse CD40, secondary ab: FITC-conjugated donkey anti-goat IgG). Whole kidney CD40 mRNA expression correlated with interstitial volume (E) but not with glomerulosclerosis or tubular cell height (data not shown). Some sections as illustrated in F showed lack of co-localization of staining, whilst there was co-localization of staining (yellow colour) (G) in other parts of the kidney. Therefore, CD40 is expressed on both F4/80 positive and negative cells in vivo. F4/80 staining alone (H) is shown for comparison. CD40 was not expressed in glomeruli (I). White arrows—glomeruli, blue arrows—double stained cells (yellow) positive for CD40 and F4/80.

Article Snippet: Soluble CD154 levels were measured in culture supernatants using the soluble CD40 ligand ELISA kit (R&D systems, MN, USA) following the manufacturer's instructions.

Techniques: Expressing, Immunohistochemical staining, Staining, Quantitation Assay, In Vivo, Comparison

Journal: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Article Title: The CD40-CD154 co-stimulation pathway mediates innate immune injury in adriamycin nephrosis.

doi: 10.1093/ndt/gfp569

Figure Lengend Snippet: Fig. 5. CD40 expression in vitro. Single cell suspensions of primary renal tubular epithelial cells (RTEC), macrophages (J774 cell line) and mesangial cells (CRL-1927 cell line) were stained for PE-CD40 or isotype control and analysed by flow cytometry. RTEC constitutively expressed high levels of CD40 (A), whereas macrophages (B) and mesangial cells (C) expressed only low amounts of CD40. Interferon-γ stimulation upregulated CD40 on macrophages (strongly) and mesangial cells (weakly) but not RTEC. Data show percentage of cells staining for PE-CD40.

Article Snippet: Soluble CD154 levels were measured in culture supernatants using the soluble CD40 ligand ELISA kit (R&D systems, MN, USA) following the manufacturer's instructions.

Techniques: Expressing, In Vitro, Staining, Control, Flow Cytometry